A comprehensive movement analysis of stair climbing can help in the research of several rehabilitation methods. This research study provides the investigations and observations of a human’s lower extremity during ascent and descent walking at different inclinations. In addition to this, the analysis of stair ascent and descent can be useful in the design of private and public environments where stairways are employed. Several normal subjects ascended and descended a flight of stairs at similar inclinations for the study. Observations such as the subject’s starting position and reaching position, handrail usage, ascending and descending speed, foot position, knee position, body position, vision, and stuttering were noted; their psychological feel on the staircase, handrail material, and their problems were also discussed during the interview.